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Anpathadon and Anviradon





We are now able to offer the best healing food supplement products for people suffering from pathogen related diseases. With Anpathadon and Anviradon, the immune system becomes at least 100 times more efficient and to date, these products have no known side effects. The product contains extracts of Andrographis Paniculata, Adhatoda vasica, Alpinia galangal, Glycyrrhiza glabra, Piper longum, Cats Claw, Lysine, Valeriana, Elderberry, ClO2-stabilised gas and distilled water.

Benefits of Anpathadon and Anviradon

Information gathered and shared with medical professionals since 2005 concludes the effect in the body when Anpathadon and Anviradon are ingested or infused.

First, Anpathadon and Anviradon destroy anaerobic and aerobic microorganisms, including bacteria, fungus, molds, yeast, and parasites that are disease-causing organisms. The exact mechanism resulting in disease-causing organisms being killed and the beneficial microorganisms saved is unknown, but results have proven this is exactly what happens. Viruses are killed by a different mechanism: Anpathadon and Anviradon prevent the formation of the special proteins of viruses, thus causing their destruction (in other words they are prevented from growing). The killing of disease-causing organisms generally happens between 4 hours and 4 weeks, but often in less than 1 week. This is true for all forms of infection and microorganisms, as well as blood diseases such as leukemia.

Second, Anpathadon oxidizes the heavy metals in the body proven by a number of tests done on the hair roots of individuals. Checked before therapy, heavy metals were present. After taking Anpathadon, the heavy metals were gone including mercury, lead, and several other metals. We know hair root testing is not totally accurate but rather a good indicator. Oxidation of the heavy metals is not the same as chelating, but the result is the same. When a heavy metal is oxidized, it is neutralized and simply washed out of the body.

Third, Anpathadon is carried throughout the body oxidizing and neutralizing foreign matters that are generally poisonous. When oxidized, poisons are neutralized and merely washed out of the body. Nearly all poisonous materials are easily oxidized. These poisons are attached at various locations and hinder the function of the body. As they are oxidized they are detached and washed out of the body.

Fourth, most forms of poisons from snakes and other venomous animals are oxidized when adequate amounts of Anpathadon and Anviradon are taken. Most food poisons found in restaurants or in your refrigerator after food has aged are oxidized, which is why one should take a number of doses when feeling the effects of food poisoning.

Fifth, the poisons generated by burns, particularly 3rd degree burns are neutralized when covered immediately (or even within hours) with Anpathadon. Anpathadon should not be left on for more than 1 to 5 minutes and must then be rinsed off with water.

The Fundamentals of Using Anpathadon

The natural substances generated by Anpathadon do not remain in the body longer than 1 to 2 hours, generally lasting a little over 1 hour. The dosing amount does not seem to make a great deal of difference compared to the amount of time that Anpathadon remains active in the body. It does not matter whether it is a large amount or small amount; it still deteriorates into little more than table salt in a little over ninety minutes. It is demonstrated over 1,000 times that small doses administered at frequent intervals, up to once per hour, are more effective than a large dose administered once or twice per day. In addressing the various methods for administering Anpathadon, keep in mind it will be much more effective to take either each hour or once every 2 hours, using smaller doses that are equal to or greater than one large dose.

The Fundamental Difference Between Viruses and Other Pathogens

Viruses are several orders of magnitude smaller than other pathogens, such as bacteria, molds, fungus, and parasites. Anpathadon and Anviradon kill viruses in a much different way than other pathogens. The other pathogens are killed by an explosive oxidative reaction of the gas, but viruses are killed because the active gas prevents the special virus proteins from forming, thus the viruses die. The larger pathogens are killed instantly; viruses are basically prevented from growing and are killed over minutes or hours. When a virus causes a disease, one must keep Anpathadon and Anviradon present in the body for an extended period, as opposed to a fast kill used for bacteria or parasites. Normally, a viral disease, such as the flu will last at least 12 hours many times much longer. We know that active gas from the product only remains present for about 1 hour. That means you must take Anpathadon every hour and Anviradon every two hours for an extended period of time to keep the viruses from forming, thus they will die. Take as much Anpathadon as one can handle without getting more sick or nauseous.

The Bodys Immune System

A pathogen is a biological agent that causes disease to its host cell. Our bodies are equipped with a natural order of defense against many of these pathogens. The human immune system, along with some friendly bacteria present in the bodys normal flora was created to combat these microorganisms.
Our bodies have three basic means to defend against an invasion of pathogens. The first is a phagocytic immune response in which white blood cells destroy foreign particles by engulfing and ingesting them. The second defense is the humeral immune response. The body sends highly specific protein antibodies into the blood stream to destroy the pathogens. And the third defense mechanism is the cellular immune response that uses T-lymphocytes. These can turn into special killer T-cells or cytotoxic T-cells able to attack pathogens. When our immune system is compromised, the pathogens normally held at bay can now proliferate and cause harm to our bodies.
In the last decade, our food and water sources and even the air we breathe have compromised our immune systems. Antibiotics and other prescription drugs prescribed by a physician can cause a myriad of problems within the immune system. For example, large amounts of antibiotics can lead to bone marrow suppression. Many of our commonly prescribed antibiotics can cause hemolytic anemia, a deficiency of red blood cells in response to certain toxins or infectious agents. Over-the-counter pain medications such as ibuprofen can cause neutropenia, an abnormal decrease in the new blood cells. Aspirin can cause GI upset, anemia and is found to exacerbate irritable bowel disease and Crohns disease.

Many illnesses are caused by immune dysfunction. A list of examples based on body systems is:
Respiratory System - cough (dry and productive), abnormal lung sounds, hyperventilation
Cardiovascular System hypotension, anemia
G.I. System colitis, diarrhea, irritable bowel disease, Crohns disease (research is showing that environmental agents such as pesticides, food additives, tobacco and radiation can bring on irritable bowel disease)
Urinary System Frequency and/or burning on urination, blood in urine
Musculoskeletal System joint, mobility, edema, pain, ataxia (loss of muscle coordination)
Skin rashes, lesions, dermatitis, edema, inflammation, sloughing of dead skin from psoriasis, eczema, seborrhea for example
Neurosensory System cognitive dysfunction, hearing loss, visual changes, headaches and/or migraines.

The importance of nutrition is gaining recognition in the health community. Vitamins are essential for cell proliferation and maturation of immune cells. Having an excess or deficiency in vitamins can lead to impaired immune function. Trace minerals such as copper, iron, manganese, selenium and zinc are essential for proper immune function.
Lifestyle factors also affect the immune system. As mentioned earlier, poor nutritional status, smoking, excessive consumption of alcohol, illicit drug use, STDs, occupational and/or residential exposure to environmental radiation and pollutants have all been associated with an impaired immune system.


The activity of a liquid solution of Anpathadon has been investigated in vitro on human and murine cancer cell lines as well as normal cells, and in vivo on C57/BL6 mice grafted with the syngeneic murine melanoma cell line B16.

IN VITRO and VIVO EFFECT
IN VITRO EFFECT


A. Cancer cell lines

The cells were seeded at 5.104 cells in 1 ml RPMI 1640 medium supplemented with 10% fetal calf serum in 12-well plates in culture at 37C in an incubator set at 5% CO2. After 24 h culture, Anpathadon was added in each well at concentrations ranging from 0 to 20 l, and incubation was carried on for two more days. Then, cells were scraped from the wells, washed with phosphate-buffered saline pH 7.4 by centrifugation, and counted after staining the cells with Trypan Blue to assess their viability and figure out the lethal dose killing 50% of the cells (LD50).

For the following cell lines, the LD 50 expressed in l of Anpathadon was :
- 1.25 l/ml for human IC8 melanoma
- 1.25 l/ml for human TW12 melanoma
- 2 l/ml for human SKN-MC neuroblastoma
- 2 l/ml for human CHP212 neuroblastoma
- 1.25 l/ml for human Burkitts lymphoma
- 1. 25 l/ml for human Jurkat T lymphoma
- 2 l/ml for mouse B16 melanoma
- 2 l/ml for mouse Neuro2A neuroblastoma

1.B. Normal cells

The same procedure was applied on normal human cells.
For the following cells, the LD 50 expressed in l of Anpathadon was :
- 150 l/ml for human lymphocytes
- 200 l/ml for human macrophages
- 150 l/ml for human keratinocytes
- 150 l/ml for human fibroblasts

C. Conclusions
Anpathadon has a very potent cytotoxic activity on any cancer cell line. The mechanism of killing seems to involve direct effect on the mitochondrial potential since only a few cells in each line displayed the markers of apoptosis, as assessed by flow cytometry. Normal cells seem almost unsensitive to the drug, but since they do not divide (macrophages) or only divide at a very slow rate (fibroblasts), their uptake of the drug is likely to be quite low as compared to cancer cells, and it is thus difficult to compare the activity on normal cells to that on the fast-dividing cancer cells. It is noteworthy that Anpathadon added at 25% in complete culture medium did not induce any sign contamination by fungus or bacteria, even after several weeks of incubation at 37C. Therefore, this drug has also a bactericidal and fungicidal activity. The sterile filtration of Anpathadon solution at 0.22 did not change anything to its cytotoxic properties.

IN VIVO EFFECT
We selected B16 murine melanoma cells to assess the effect of Anpathadon because of the high sensitivity of these cells to the cytotoxic activity of the drug in vitro. The growth of tumors induced by B16 melanoma cells grafted in vivo in C57/BL6 mice is well documented in the scientific literature. The tumors induced by a subcutaneous injection of 2.105 cells usually kill the mice within one month and a half.

B16 melanoma cells were grown in culture for three days in complete medium at 37C, then scraped in sterile conditions and counted. 2.105 B16 melanoma cells were taken up in 100 l of phosphate-buffered saline pH 7.4 and injected subcutaneously in the right flank of C57/BL6 mice aged 6 weeks (Charles River Laboratories) kept in an animal facility in compliance with the rules established by the University of Lyon-1.

After three days, the mice (groups of five) were treated by daily subcutaneous injections, with a tenfold dilution of Anpathadon, in the opposite flank of volumes ranging from 0 l for the control mice to 150 l. The injected volumes were respectively 0, 10 l, 25 l, 50 l, 75 l, 100 l, 125 l, 150 l. The tumor size was measured once on every other day for one month.

The results are summarized in the following table and the graph showing the mean volume of tumors as a function of the treatment volume.

Daily treatment

(l) for 1 month

Mean tumor volume

(mm3) SE

Number of mice

bearing tumors

0

6540 2480

5/5

10

4160 1570

4/5

25

1330 810

2/5

50

3270 1420

3/5

20

860 410

1/5


It appears that the treatment with high amounts of Anpathadon above 50 l leads to the facilitation of tumor growth. One likely explanation of the results is an alteration by the drug of the immune system that becomes unable to control the proliferation of the tumor cells. However, low doses seem to be effective. In the group of mice treated with daily injections of 20 l, tumors were seen in only 1 mouse out of 5. The efficacy of the treatment with Anpathadon of mice grafted with tumor cells cannot give much information on a similar treatment applied to humans. The tumors cells injected in mice are tremendously more aggressive than human tumor cells and they divide at a very fast rate. The doubling time is about four days for the tumors induced in mice with the B16 melanoma cells, whereas it ranges from two to six months for the most prevalent human tumors.

CONCLUSIONS
Although Anpathadon is highly cytotoxic for the human and murine tumors cells in vitro, it is difficult to assess accurately its efficiency in treating tumors in vivo. Most syngeneic murine tumors cells are very aggressive and grow at a fast rate. There is no information in the scientific literature about any treatment that would completely abolish melanoma tumor growth in mice following injection of 2.105 cells. Therefore, blocking tumor growth for 80% of the mice injected daily with 20 l is a very satisfactory result. Moreover, the sizes of the tumors grown in one mouse out of five in this group were small as compared to the controls. It is noteworthy that all mice showing no sign of tumor after a month remained tumor-free for the next two months, thus showing that all tumor cells were killed by the treatment.

Five days after the subcutaneous injection of 2.105 B16 melanoma cells in mice, the tumor induced at the injection site is estimated to be 10 mm3. The corresponding amount in a human patient would be about 40 g. Therefore, keeping some mice tumor-free is already a very interesting result. Of course, it would be nice to cure mice in which the tumors are already detectable, but a small subcutaneous tumor of 125 mm3, that is barely detectable in mice, would correspond to a 800 g tumor in a human weighing 80 kg. With such an amount of tumors in a patient, obtaining a total cure seems to be a hopeless task.

It should be safe to treat human beings with low volumes of Anpathadon, but more research is needed in mice In order to improve the results and eventually obtain a total cure. The major unsolved question regarding Anpathadon is its influence on the immune system. There must be an equilibrium state between the effect of the drug on the immune cells and its effect on the tumor cells. This point requires some further work with mice to be fully understood. Then it will be feasible to set up an optimized procedure of treatment applicable to human patients.

Made in Lyon, France, August 24, 2012
Laboratory of Dermatological Research
University of Lyon-1
Lyon, France
Faculty of Pharmacy
University of Paris XI
Chatenay-Malabry, France

UPDATED REPORT ON THE ANTITUMORAL
ACTIVITY OF ANPATHADON

The activity of Anpathadon was tested after addition of artemisinin up to 5% final concentration. Artemisinin was dissolved in DMSO before addition to the solution of Anpathadon.
The antitumoral effect was tested on human melanoma cell lines IC8 and TW12 as described. The cells were seeded at 5.104 cells in 1 ml RPMI 1640 medium supplemented with 10% fetal calf serum in 12-well plates in culture at 37C in an incubator set at 5% CO2. After 24 h culture, Anpathadon was added in each well at concentrations ranging from 0 to 20 l, and incubation was carried on for two more days. Then, cells were scraped from the wells, washed with phosphate-buffered saline pH 7.4 by centrifugation, and counted after staining the cells with Trypan Blue to assess their viability and figure out the lethal dose killing 50% of the cells (LD50). On both cell lines, the LD 50 dropped from 1.25 ml/ml to 0.75 ml/ml. An experiment on normal lymphocytes did not show any difference and the LD 50 was at 150 ml/ml with or without artemisinin.

The results suggest that the addition of artemisinin significantly enhances the antitumoral effect of Anpathadon.

Made in Singapore, January 8, 2013

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